DS CEN ISO/TS 15216-2:2013

Mikrobiologjia e ushqimit dhe ushqyerja e kafshës - Metodat horizontale për përcaktimin e Hepatit A dhe norovirusit në ushqim duke përdorur RT-PCR në kohë reale - Pjesa 2: Metoda për zbulimin kualitativ (ISO/TS 15216-2:2013, Corrected Version 2013-05-01)

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/TS 15216-2:2013, Corrected Version 2013-05-01)

Scope

ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.