ISO/PRF 5132

Animal and vegetable fats and oils — High-performance liquid chromatography (HPLC) analysis of phenolic antioxidants

Scope

This method can be used to quantify propyl gallate (PG), octyl gallate (OG), dodecyl gallate (also called lauryl gallate) (LG), 2,4,5-trihydroxybutyrophenone (THBP), tert-butylhydroquinone (TBHQ), nordihydroguai¬aretic acid (NDGA), 2- and 3-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHT Alcohol or Ionox-100), and/or 2,6-di-tert-butyl-4-hydroxytoluene (BHT) in oils, shortenings and animal fats at concentrations normally added to oils. The method can also be used be used to determine absence of these compounds from oils within the limitations of the test. Applicable to animal and vegetable fats, oils and shortenings. The sample is diluted in hexanes, antioxidants are extracted into acetonitrile, diluted with isopropanol and analyzed by reversed-phase gradient HPLC with UV detection at 280 nm.