DS CEN/TS 17883:2022

Environmental characterization of leachates from waste and soil using reproductive and toxicological gene expression in Daphnia magna

Publication date: Oct 31, 2023

General information

Current stage: 60.60 Effective date: Oct 31, 2023

Originator: DPS

Owner: DPS/KT 230

Type: Technical Specification

ICS: 13.030.01 13.060.70 13.080.99

Languages: English

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Status: Published

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This document specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to quantify the abundance of specific mRNA molecules extracted from Daphnia magna. The method allows the identification of molecular responses to exposures for potentially toxic substances through the analysis of the abundance of specific mRNA molecules. In this document, the central genes involved in reproductive and toxic responses are included.

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DS CEN/TS 17883:2022
60.60 Standard published
Oct 31, 2023