SSH EN 17298:2019

Animal feeding stuffs: Methods of sampling and analysis - Determination of benzoic and sorbic acid by High Pressure Liquid Chromatography (HPLC)

Scope

This document specifies a method for the determination of benzoic acid and sorbic acid in animal feeding stuffs by high-performance liquid chromatography method with ultra-violet detection (HPLC-UV).
The method is intended to be used for the determination of benzoic acid and sorbic acid as active substances in feed additives, premixtures and compound feed and for benzoic acid in water. This method determines the total extractable concentration of these organic acids and their salts.
The working range of the method must be determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. A working range between 5 mg/l and 100 mg/l should be accessible.
The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 9,0 %.
On the basis of the referred working range, sample weigh and extraction volume, limits of quantification (LOQ), as calculated (Table 1) on the basis of a wavelength of 230 nm, should be achievable.