ISO/CD 22699

Premature Chromosome Condensation (PCC) for biological dosimetry

Scope

The purpose of this International Standard is to provide criteria for quality assurance and quality control, evaluation of the performance and the accreditation of biological dosimetry by cytogenetic service laboratories using the PCC.
This International Standard addresses:
1) the confidentiality of personal information, for the customer and the laboratory;
2) the laboratory safety requirements;
3) the calibration sources and calibration dose ranges useful for establishing the reference dose-effect curves that contributes to the dose estimation from the frequency of chromosome damage and the minimum resolvable doses;
4) the scoring procedure for chromosome damage using premature chromosome condensation used for evaluation of exposure;
5) the criteria for converting a measured chromosome damage into an estimate of absorbed dose;
6) the reporting of results;
7) the quality assurance and quality control;
8) informative annexes containing sample instructions for customer, sample questionnaire, sample datasheet for recording damage, sample of report and fitting of the low dose-response curve by the method of maximum likelihood and calculating the error of dose estimate.