SSH EN 17270:2019

Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography

Publication date: Jun 25, 2020

General information

Current stage: 60.60 Effective date: Jan 31, 2020

Originator: DPS

Owner: DPS/KT 275

Type: European Norm

ICS: 65.120

Languages: English

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Status: Published

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Scope

This document method is applicable for the determination of theobromine in compound feed by liquid chromatography with UV detection in the tested range of 27 to 307 mg/kg. This method has been validated using complementary compound feed for adult dogs and complementary compound feedstuff for horses. The actual working range may extend beyond the tested range. Alternative chromatography conditions using liquid chromatography tandem mass spectrometry (LC-MS/MS) are also provided for the validated range of 49 to 307 mg/kg. This method has also been shown to be fit for purpose for the determination of theobromine in baking chocolate by both HPLC-UV and LC-MS/MS.

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PUBLISHED
SSH EN 17270:2019
60.60 Standard published
Jan 31, 2020

Related project

Adopted from prEN 17270

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