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ISO 17601:2016

Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

Jan 12, 2016

General information

90.92     Oct 24, 2023

ISO

ISO/TC 190/SC 4

International Standard

13.080.30  

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Scope

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Life cycle

NOW

PUBLISHED
ISO 17601:2016
90.92 Standard to be revised
Oct 24, 2023

REVISED BY

IN_DEVELOPMENT
ISO/FDIS 17601

National adoptions

SSH EN ISO 17601:2018  
DPS

Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016)

60.60 Standard published

DPS/KT 230 more