DS ISO/TS 21569-6:2016

Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences

Publication date: Sep 28, 2017

General information

Current stage: 60.60 Effective date: Aug 8, 2017

Originator: DPS

Owner: DPS/KT 275

Type: Technical Specification

ICS: 67.050

Languages: English

Buying

Status: Published

PDF - ALL 2,261

Paper - ALL 2,261

CD - ALL 2,261

PDF and Paper - ALL 4,522

Language

Language in which you want to receive the document.

Scope

ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modified cry1Ab/Ac gene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin promoter (Pubi) and the cry1Ab/Ac gene. The modified cry1Ab/Ac gene and the Pubi-cry construct are frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.
ISO/TS 21569-6:2016 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

Life cycle

NOW

PUBLISHED
DS ISO/TS 21569-6:2016
60.60 Standard published
Aug 8, 2017

Related project

Adopted from ISO/TS 21569-6:2016

Preview

Only informative sections of projects are publicly available. To view the full content, you will need to members of the committee. If you are a member, please log in to your account by clicking on the "Log in" button.